Association between the FABP2 Ala54Thr, PPARα Leu162/Val, and PPARα intron7 polymorphisms and blood lipids ApoB and ApoCIII in hypertriglyceridemic subjects in Tehran

Pishva, Hamideh; Mahboob, Soltan Ali; Mehdipour, Parvin; Eshraghian, Mohammad Reza; Mohammadi-Asl, Javad; Hosseini, Saeed; Rahmany, Mazaher

doi:10.1016/j.jacl.2009.04.001

« Previous Next »Journal of Clinical Lipidology
Volume 3, Issue 3 , Pages 187-194, May 2009

Association between the FABP2 Ala54Thr, PPARα Leu162/Val, and PPARα intron7 polymorphisms and blood lipids ApoB and ApoCIII in hypertriglyceridemic subjects in Tehran

Hamideh Pishva, PhD
- Department of Nutrition and Biochemistry, School of Public Health and Institute of Public Research, Tehran University of Medical Sciences, Tehran, Iran
Soltan Ali Mahboob, PhD
- Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
- Corresponding author.
Parvin Mehdipour, PhD
- Department of Medical Genetic, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Reza Eshraghian, PhD
- Department of Epidemiology and Biostatistics, School of Public Health and Institute of Public Research, Tehran University of Medical Sciences, Tehran, Iran
Javad Mohammadi-Asl, MS
- Department of Genetics, School of Medicine, Ahvaz Jondishapur University of Medical Sciences, Ahvaz, Iran
Saeed Hosseini, MD, PhD
- Endocrinology Metabolism Research Center (EMRC), Tehran University of Medical Sciences, Tehran, Iran
Mazaher Rahmany, BS
- Endocrinology Metabolism Research Center (EMRC), Tehran University of Medical Sciences, Tehran, Iran

Received 4 September 2008; accepted 13 April 2009. published online 20 April 2009.

Background

The alanine to threonine substitution at codon 54 in the FABP2 gene and PPARα Val162 allele have been associated with hypertriglyceridemia.

Objective

We sought to determine the prevalence of the Ala54Thr polymorphism of fatty acid binding protein (FABP) 2 gene and the Leu162/Val in exon 5 and G/C in intron7 polymorphism of peroxisome proliferator-activated receptor alpha (PPARα) gene in hypertriglyceridemic patients and their associations with blood lipid concentrations.

Methods

A total of 170 hypertriglyceridemic subjects were enrolled and genotyped for Ala54Thr, Leu162Val, and intron 7 polymorphism by the use of a polymerase chain reaction-restriction fragment length polymorphism method. Fasting blood triglyceride, total cholesterol (TC), low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, apolipoprotein (Apo)B, and ApoCIII also were determined.

Results

We found frequency of 81.2% for the Thr54 polymorphism among hypertriglyceridemic subjects. Positive associations were observed between this polymorphism and greater blood triglyceride, very low-density lipoprotein, and ApoCIII levels and lower blood high-density lipoprotein cholesterol concentration both in men and women. However, no association was found between the Thr54 polymorphism and TC, low-density lipoprotein cholesterol, ApoB, and body mass index. Frequency of the Leu162Val polymorphism was 21.8%. The Leu162Val polymorphism was not associated with lipid and lipoprotein concentrations in hypertriglyceridemic subjects (both in men and women). The frequency of intron7 polymorphism was 55.3% in subjects studied and, except for body mass index and TC, no association was found between the intron7 allele and blood lipids ApoB, and ApoCIII.

Conclusion

Frequency of the Thr54 polymorphism is high in hypertriglyceridemic subjects, and the presence of this allele may increase some blood lipid and lipoprotein concentrations. In addition, the frequency of intron7 polymorphism may be greater than Leu162Val in hypertriglyceridemic patients.

Keywords: FABP2, PPARα, Intron7, Polymorphism, Hypertriglyceridemia, Blood lipids, ApoB, ApoCIII, HDL-C, VLDL